Gene Doping Workshop in Japan

Chaired by Teruaki Tozaki, Ph.D.

Organized by the Genetic Analysis Department, Laboratory of Racing Chemistry, Japan

Supported by the IFHA Gene Doping Control Subcommittee, the AORC Gene Doping ad-hoc committee, and the ISBC

Day: September 26 and 27, 2019

Venue: Utsunomiya Tobu Hotel Grande

https://www.tobuhotelutsunomiya.com/

Backgrounds

The development of veterinary sciences contributes to the health and welfare of racehorse, but also may create concerns of genetic doping as a new threat. In 2017, the Gene Doping Control Subcommittee was established at the IFHA to discuss the definition of gene therapy in the horse racing industry and the genetic doping for its misuse. In the AORC and ISBC, special committees were also established with discussion of genetic doping and genetically modified racehorse concerns.

Workshop Principles

  • Participants understand what genetic doping is and experience its detection method.
  • Look at the genetic laboratory and experience actual gene doping detection. Participants understand which types of analysts and which instruments are required for gene doping detection.
  • Success by any one of us is success for racing industries and all of us. Developing gene doping testing methods is a large undertaking. Therefore, cooperative and collaborative activities are essential to accomplish our goals.

Program (Tentative)

Morning, 26th September 2019

  • General greeting
    • Greeting from Japan Racing Association
    • Greeting from Laboratory of Racing Chemistry
  • Plenary
    • Gene Doping Detection in human sports (invited speaker)
    • Gene Doping Detection in human sports (invited speaker)
  • General presentation
    • IFHA Gene Doping Updates
    • AORC Gene Doping Updates
    • Activities in Australia
    • Activities in Japan (Instruction of gene doping detection)
      The other presentations

Afternoon, 27th September 2019

Laboratory tour & Demonstration of gene doping detection
  • Preparation of reference material for transgene detection
  • Sample banking system
  • DNA extraction from plasma and urine
  • Real-time PCR detection of multiple targets using microfluidic real-time PCR (Screening test)
  • Absolute quantitative detection of gene doping materials using droplet digital PCR (Confirming test)
  • Detection of genetically modified regions using a next generation sequencer (NGS) with bioinformatics analyses.
  • Detection of therapeutic nucleotides by mass spectrometry.

Teruaki TOZAKI, Ph.D.

Laboratory of Racing Chemistry, Japan

Contacts:
ttozaki@lrc.or.jp
gmd_iod02@or.knt.co.jp

Microfluidic real-time PCR

Multiplex real-time PCR detection of transgenes (screening tests)

Droplet digital PCR

Absolute Quantitative PCR detection of transgenes (confirming tests)

QX200TM AutoDG Droplet DigitalTM PCR System (Bio-Lad)

QX200TM Droplet Generator System (Bio-Lad)

(©2019 Bio-Rad Laboratories, Inc. All rights reserved.)

https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3815-6

https://www.mdpi.com/2073-4425/10/3/243

Next Generation Sequencer & Bioinformatics analyses

Detection of genetically modified animals

Originally developed software

  • DopSeq: detection of inserted transgenes
  • Contradiction Counter: parentage verification at whole genome SNP and INDEL

(Collaborated with the Amelieff Corporation, http://amelieff.jp/english/)

A quadrupole-time-of-flight (Q-TOF) tandem mass spectrometer

Detection of therapeutic nucleotides

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